Iterative expansion microscopy
نویسندگان
چکیده
منابع مشابه
Expansion microscopy
In optical microscopy, fine structural details are resolved by using refraction to magnify images of a specimen. Here we report the discovery that, by synthesizing a swellable polymer network within a specimen, it can be physically expanded, resulting in physical magnification. By covalently anchoring specific labels located within the specimen directly to the polymer network, labels spaced clo...
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In this paper we describe an improvement to the popular IDA* search algorithm that emphasizes a different spacefor-time trade-off than previously suggested. In particular, our algorithm, called Iterative-Expansion A* (IEA*), focuses on reducing redundant node expansions within individual depth-first search (DFS) iterations of IDA* by employing a relatively small amount of available memory— boun...
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Until the past decade, optical microscopy of biological specimens was strongly limited by diffraction and scattering, affecting imaging resolution and depth, respectively. Now, numerous methods are available to overcome each of these limitations, but sub-diffraction limited resolution imaging over large volumes of scattering tissue is still a challenge. This work concerns the development of a n...
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Many neuroscience questions center around understanding how the molecules and wiring in neural circuits mechanistically yield behavioral functions, or go awry in disease states. However, mapping the molecules and wiring of neurons across the large scales of neural circuits has posed a great challenge. We recently developed expansion microscopy (ExM), a process in which we physically magnify bio...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2017
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth.4261